The latest version, dubbed Carbon, is lightweight and highly customizable.
The second problem with the design above was the duplication of code for some of the infrastructure components. Don’t get me wrong, not all duplication is bad (as Rob Pike said: “A little copying is better than a little dependency”). But in this case it’s a significant piece of logic - a custom implementation of a TCP protocol in one case, and filtering of high-throughput data in the second. That’s why we will add the following standalone modules:
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In the best scenario, removing a feature, like e.g. the Dispatching Firmware responsibility from our example, could be as easy as removing modules which are responsible for it, in our example it would be: “FirmwareDispatcher” and “FirmwareDispatcher.Infra” (and their wiring in the Entrypoint module).
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In 2010, ONT combined two technologies addressing each of the main outstanding problems. The first was an engineered nanopore developed in collaboration with Bayley’s lab that could discriminate between individual DNA bases, solving the resolution issue. The second was a trick to slow the DNA down to detectable speeds, using the familiar DNA polymerase enzyme. Mark Akeson’s lab at UC Santa Cruz had identified a specific polymerase from the bacterial virus ɸ29 that replicated DNA at an ideal speed for detection via nanopore. Template DNA strands were replicated just before entering the nanopore, passing through slowly enough for individual bases’ effect on the electrical current to be detectable and allowing the DNA sequence to be read one base at a time.